Adaptive Wavefront Correctionin Two-photon Microscopy
Rückel, Markus
The focus of a two-photon microscope is often degraded by inhomogeneities in the
refractive index within biological specimens. In this book it is shown for various
specimens, even for living zebrafish, that the resolution and the fluorescence signal of a
two-photon microscope can be substantially improved by using adaptive optics, i.e.
wavefront correction based on coherence-gated wavefront sensing (CGWS). The advantage
of using CGWS relies on...