Covering the key techniques used when working with fungal plant pathogens, this practical manual deals with recognition of disease symptoms, detection and identification of fungi and methods to characterize them well as curation, quarantine and quality assurance. This new edition includes updates with respect to: * Greater awareness and concern internationally about plant health and biosecurity* Molecular biology - next generation sequencing and in field detection* Improved opportunities for surveillance and detection in substrates, such as remote sensing* Changes in taxonomy and reference to more current fungal plant pathogens* New chapters on tree health, public awareness, outreach, and communications