yi dan bai mei yi zhi yin zi TIs wei gan shu kuai gen zhong zhu yao chu cang xing dan bai zhi ben yan jiu yi trypsin Sepharose 4B qin he guan zhu ceng xi fang fa cong tai nong 57 hao gan shu kuai gen he tai nong 65 hao gan shu Ipomoea batatas L Lam kuai gen zhong chun hua chu TIs jie you yi xi lie kang yang hua DHR123 huo xing shi yan ru qing chu DPPH zi you ji qing chu hydroxyl zi you ji huan yuan li de ce ding qing chu chao yang zi you ji de ce ding kang di mi du zhi dan bai yang hua yi zhi peroxynitrite yang hua he bao hu DNA yang hua deng yan jiu du xian shi TIs ju you kang yang hua huo xing wo men jing you iodoacetamide xiu shi ban guang an suan can ji ji yi NBSI N bromosuccinimide xiu shi se an suan ce lian hou jin xing kang yang hua huo xing shi yan fa xian TIs zhong ban guang an suan can ji he se an suan ce lian fen bie dui qing chu DPPH zi you ji ji qing chu hydroxyl zi you ji you ming xian ying xiang qi zhong ban guang an suan can ji dui qing chu DPPH zi you ji de kang yang hua huo xing you hen da de ying xiang er wo men fen bie yi wei dan bai mei pronase E he wei dan bai mei shui jie chan wu zai yi ning ru yi dan bai mei shui jie TIs fen bie zuo qing chu DPPH zi you ji dui kang di mi du zhi dan bai yang hua le jie qi bu tong kang yang hua huo xing zai jiang TIs shui jie chan wu yi Sephadex G 50 jin xing guan zhu ceng xi fen li bing jiang fen li de shui jie chan wu jin xing qing chu hydroxyl zi you ji huo xing shi yan jie guo xian shi xiao fen zi sheng ran reng ran ju you qing chu hydroxyl zi you ji neng li