In the past decade, a tremendous progress in understanding the function of carbohydrates or glycans took place. This progress also starts showing the promise of drug development based on the function of carbohydrates. This critical progress largely owes a development of new methodology to determine a role of a carbohydrate and then develop a method to interfere such biological process, mostly taking a form of protein-carbohydrate interaction. In the current three volumes of Methods in Enzymology focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers¿ own research efforts. Each chapter has been designed so that enough scientific background will be given in each chapter for further development of methods by readers themselves. The books thus should be useful for all levels of scientists starting from the last years of colleges, graduate students, postdoctoral fellows to professors and to all levels of scientists in research institutes including industry. The editor is highly hopeful and confident that these volumes of Methods in Enzymology will contribute to another surge in determining the structure and function of glycans and developing new drugs based on carbohydrate functions.