This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry, Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids, Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins, Biochemical Analysis of Phospholipase D., Measurement of Autotaxin/Lysophospholipase D Activity, Platelet-Activating Factor, Quantitative measurement of PtdIns(3, 4, 5)P3, Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes, Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins, Biochemical Analysis of Inositol Phosphate Kinases, Analysis of the phosphoinositides and their aqueous metabolites, Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism, Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo, A rapid and sensitive method to measure secretion of sphingosine-1-phosphate, Ceramide Kinase and Ceramide-1-Phosphate, Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells, Lipid Phosphate Phosphatases from Saccharomyces cerevisiae.