This second edition provides new and update chapters on different aspects and applications on plants, algae, photosynthetic bacteria, synthetic construct design, and multiplex cloning. Chapters cover multiple aspects of synthetic metabolic, photosynthetic systems, metabolic and signalling pathways, advanced engineering of metabolic networks, isolation of organelles and co-culture of microorganisms, and methods for the on-command manipulation of the relative stability of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Plant Synthetic Biology: Methods and Protocols aims to be a useful resource for both researchers starting to explore novel experimental avenues as well as for experts willing to expand their portfolio of tools and strategies.