The Chlamydomonas Sourcebook, Volume One, Third Edition: Introduction to Chlamydomonas and Its Laboratory Use has been fully revised and updated to include a wealth of new resources for the Chlamydomonas community (new mutant libraries, new omics studies, and potentially more information about different Chlamydomonas species in the environment). In addition to updates on molecular techniques and analysis of the sequenced genome, the book presents the latest in research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, and more.