This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis, antha-guided automation of Darwin assembly for the construction of bespoke gene libraries, rapid cloning of random mutagenesis libraries using PTO-Quickstep, and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.
Lieferbar
ISBN | 9781071621516 |
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Sprache | eng |
Cover | Fester Einband |
Verlag | Springer Us |
Jahr | 20220622 |
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